Journal: Alzheimer's & Dementia
Article Title: Interconnections of insulin/IGF‐1 signaling and autophagy abnormalities in Alzheimer's disease
doi: 10.1002/alz.70099
Figure Lengend Snippet: Measuring endocytic and autophagic factors by FICC. A, Schematic of endocytosis (red markers) and autophagy (green markers). B, C, Images from light microscopy (B, upper panels) and corresponding CellProfiler (B, lower panels), or confocal microscopy (C) in HFF1 cells. Images show LC3 and LAMP2 in green and RAB5 and RAB7 in red fluorescence for four different combinations: LC3/RAB5, LC3/RAB7, LAMP2/RAB5, and LAMP2/RAB7. Nuclei were counterstained with DAPI. The insets in (C) depict magnifications from YZ or XZ planes as indicated. Size bars: 5 µm in (B), 20 µm in (C). For visualization purposes, the colors in the CellProfiler images (B) have been amplified. D, CellProfiler data on speckles counts per area and sizes for LC3, LAMP2, RAB5, and RAB7 for naïve AD, OC, and YC cells. Data are from 200 cells per group. One‐way analysis of variance with Sǐdák multiple comparisons was used for group comparisons depicting ns < 0.1; * P < 0.05; ** P < 0.01; *** P < 0.001. AD, late‐onset Alzheimer's disease patient; HFF1, human foreskin fibroblast 1; FICC, fluorescence immunocytochemistry; LAMP2, lysosomal associated membrane protein 2; LC3, microtubule‐associated proteins 1A/1B light chain 3; LOAD, late‐onset Alzheimer's disease; OC, non‐demented age‐matched control; RAB5, Ras related protein 5; RAB7, Ras related protein 7; YC, healthy young control.
Article Snippet: The human foreskin fibroblast 1 (HFF1) cell line was purchased from the American Type Culture Collection (SCRC‐1041).
Techniques: Light Microscopy, Confocal Microscopy, Fluorescence, Amplification, Immunocytochemistry, Membrane, Control
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